ELAVL4 Knockout cell line (H1)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

ELAVL4 Knockout cell line (H1) Order

Catalog#

YKO-H989

Size

2*10^5

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ELAVL4 Knockout cell line (H1)

Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

ELAVL4

Gene ID

1996

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

Target region

Knockout region: Exon 1 (based on transcript ELAVL4-205)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:H1			STR Info (Cell bank) Cell Line:H1.NPC-i3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	9	11		9	11
D13S317	8	11		8	11
D7S820	8	12		8	12
D16S539	9	13		9	13
VWA	15	17		15	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	12	13		12	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ELAVL4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ELAVL4 gene knockout ELAVL4 Knockout cell line (H1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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